Benefits of biophysical tests of saliva for athletic functionality rating tests

Teoriya i praktika fizicheskoy kultury №7 2019

A.M. Vyshedko1
PhD L.V. Stepanova1
Dr.Biol., Associate Professor O.A. Kolenchukova1, 2
Dr.Biol., Professor V.A. Kratasyuk1, 3
1Siberian Federal University, Krasnoyarsk
2Research Institute of Medical Problems of the North, Krasnoyarsk
3Institute of Biophysics SB RAS, Krasnoyarsk

Modern physical training systems give a high priority to the training process individualizing elements that may be efficient enough only when supported by objective tests to rate the bodily responses to physical workloads. Moreover, success in modern professional sports is impossible unless supported by the newest medical/ biological findings and technologies in the health rating and forecast domains, with a special role played by the modern biophysical research of the cellular- and molecular-level biological processes. The study was designed to assess benefits of biophysical analysis of saliva for the athletic functionality rating tests. Sampled for the purposes of the study were athletes of different skill levels (from Class I to Honorary Masters of Sport) versus the untrained students, with the saliva sampled prior to and after physical trainings by bioluminescence and chemoluminescence test methods. The test data were processed by Statistica 10 (made by StatSoft Inc., the US) toolkit to produce median values (Me) and inter-quartile distribution (С25-С75 percentile) rates. The Mann-Whitney nonparametric U-test of the null hypothesis was used to rate differences of the independent variables, with the statistical difference rated meaningful at p<0.05.
The bioluminescence tests found the higher ferment activity of saliva in the athletes’ versus untrained students, with the ferment activity in the bioluminescence tests also tested to grow after physical workloads in the highly-skilled athletes and fall in the untrained students. The study found the saliva antioxidant system functional intensity being athletic skills dependent. Therefore, the study has demonstrated benefits of the biophysical (bioluminescence and chemoluminescence) tests of saliva for the athletic functionality rating tests in the physical workload design and management initiatives.

Keywords: elite sports, saliva, bioluminescence test, chemoluminescence test, residual luminescence, antioxidant activity rate.

References

  1. Afonshin V.E., Polevshchikov M.M., Rozhentsov V.V. Individualizatsiya fizicheskoy nagruzki [Individualization of physical activity]. Sovremennyie problemy nauki i obrazovaniya. 2016. no. 2. P. 240.
  2. Belskaya L.V., Golovanova O.A., Turmanidze V.G., Shukaylo E.S. Perspektivyi ispolzovaniya rezultatov analiza slyunyi pri planirovanii trenirovochnogo rezhima sportsmenov [Prospects for use of results of saliva analysis in athlete's training mode design]. Omskiy nauchny vestnik. 2011. no.6. pp.. 175– 178.
  3. Vinnik Yu.S., Savchenko A.A., Peryanova O.V. et al. Klinicheskie aspekty khemilyuminestsentnykh analizov [Clinical aspects of chemiluminescent analysis]. Sibirskoe meditsinskoe obozrenie. 2006. v. 3. no.  40. pp. 3-6.
  4. Elikov A.V., Galstyan A.G. Antioksidantny status u sportsmenov pri vyipolnenii dozirovannoy fizicheskoy nagruzki i v vosstanovitelnom periode [Antioxidant status in athletes during graded exercise and recovery]. Voprosy pitaniya. 2017. v. 86. no. 2. pp. 23-31.
  5. Pavlov S.E., Cherenkov D.R., Pavlova T N., Davydov A.P., Pavlov A.S. Printsipyi organizatsii kompleksnogo pedagogicheskogo i mediko-biologicheskogo testirovaniya kvalifitsirovannykh sportsmenov [Principles of organization of complex educational and biomedical testing of skilled athletes]. Detskiy trener. 2014. no.1. pp. 28-40.
  6. Stepanova L.V., Vyshedko A.M., Kolenchukova O.A. et al Ispolzovanie biolyuminestsentnogo testirovaniya slyuny v otsenke fizicheskoy podgotovlennosti sportsmenov raznoy kvalifikatsii [Bioluminescent saliva testing to estimate physical fitness of athletes of different qualifications]. Sibirskoe meditsinskoe obozrenie. 2017. no. 6. pp. 63-69.
  7. Gröschl M. Saliva: a reliable sample matrix in bioanalytics. Bioanalysis. 2017. Vol. 9. No. 8. pp. 655-668.
  8. Pingitore A., Lima G.P., Mastorci F., Quinones A., Iervasi G., Vassalle C. Exercise and oxidative stress: potential effects of antioxidant dietary strategies in sports. Nutrition. 2015. Vol. 31. No 7-8. pp. 916- 22.